科研之友
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摘要
Injury to the mature primate and subprimate optic nerve results in irreversible impairment and loss of vision, because the retinal ganglion cells (RGCs) fail to regenerate their cut axons within the optic nerve interior. This study was performed to examine whether aging monkey RGCs retain the ability to regenerate their axons in organ culture and whether axonal regeneration is associated with specific proteomic profile. Retinal stripes obtained from marmoset eyes ( C. jacchus) were cultured between the day of birth and adult stages on different substrates like laminin-1, laminin-2, collagen, matrigel and poly-D-lysine. No neurotrophic factors were added to the medium. Axonal growth was monitored with microscopy and immunohistochemistry. Onset and rate of growth was examined with time-lapse videography. Vigorous regeneration of axons occurred from identifiable morphological types of RGCs throughout all stages of life, although the numbers of axons decreased with age. Axonal growth occurred virtually only on laminin-1. Growth correlated with re-expression of the laminin-1 receptor alpha 6-integrin and sustained staining for GAP-43 as shown by immunohistochemistry and immunoblotting. At proteomic level, there is a maturation-dependent change in the protein immunostaining within the retina. When retinal slices of the same age were compared, regeneration-specific protein staining included calmodulin, fatty acid binding protein, alpha-crystallin, IFN-gamma, cyclin-dependent kinase inhibitor (p21), beta-hemoglobin, 60s-ribosomal protein, GAP-DH and ADP-ribosylation factor (ARF). To our knowledge these data are the first from subhuman animals to suggest that axonal regeneration of injured RGCs is correlated to expression of identifiable proteins within the retina.
- 出版日期2008
