Molecular characteristics of the porcine DLK1 and MEG3 genes

作者：Li X P; Do K T; Kim J J; Huang J; Zhao S H; Lee Y; Rothschild M F; Lee C K^*; Kim K S

来源：Animal Genetics, 2008, 39(2): 189-192.

DOI：10.1111/j.1365-2052.2007.01693.x

• DLK1
• GTL2
• imprinting
• MEG3
• pig
• polar overdominance
• single nucleotide polymorphism

Imprinted genes play important roles in embryo survival and postnatal growth regulation. The DLK1 and MEG3 (previously GTL2) genes are linked and reciprocally imprinted in several mammals, but their imprinting status is still unknown in pigs. In this study, we report polymorphisms, imprinting status and QTL analyses of the porcine DLK1 and MEG3 genes. Muscle and adipose DNA and RNA samples from 30-day-old animals generated with reciprocal crosses between the Korean native pig (KNP) and Yorkshire breeds were used to analyse DLK1 and MEG3 variation and expression. The samples exhibited paternal expression of DLK1 and maternal expression of MEG3 in pigs. These results indicated that the imprinting status of the DLK1 and MEG3 genes is conserved across mammalian species. By linkage analyses, we assigned the DLK1 and MEG3 genes to the telomeric region of SSC7. By QTL analyses, we confirmed a significant polar overdominance (POD) effect in DLK1, which was previously detected for several growth traits in pigs. However, no significant POD effect was found with the MEG3 locus.

• 出版日期2008-4
• 单位华中农业大学