Anti-inflammatory effect of quinoline alkaloid skimmianine isolated from Ruta graveolens L.

作者：Ratheesh M; Sindhu G; Helen Antony^*

来源：Inflammation Research, 2013, 62(4): 367-376.

DOI：10.1007/s00011-013-0588-1

• In-vivo inflammation
• Inflammatory models
• Inflammation
• Oxidative stress

The present study evaluates the anti-inflammatory effect of the quinoline alkaloid skimmianine (SKM), isolated from Ruta graveolens L., against carrageenan-induced acute inflammation.
SKM at a dose of 5.0 mg/kg body weight was found to be the minimal concentration for maximal edema inhibition. Carrageenan suspension was administered into the sub-plantar tissue of the right hind paw 1 h after SKM and diclofenac (20 mg/kg) administration (i.p.). Paw edema was determined 3 h after carrageenan administration. The rats were then killed and mRNA expressions of TNF-alpha and IL-6, levels of PGE(2) and TBARS, activities of COX-2, 5-LOX, SOD, catalase, glutathione peroxidase (GPx) and myeloperoxidase (MPO) and the level of nitrite were measured.
SKM treatment resulted in a decrease in the mRNA levels of TNF-alpha and IL-6, which are upstream events of the inflammatory cascade. The levels of PGE(2) and NO and the activities of COX-2 and 5-LOX were also significantly reduced after SKM treatment. Neutrophil infiltration, lipid peroxidation and associated oxidative stress in the paw tissue were reduced following SKM treatment.
These results support the anti-inflammatory properties of skimmianine and its multi-targeted mechanism of action, suggesting its potential therapeutic efficacy in various inflammatory diseases.

• 出版日期2013-4