Adipocyte enhancer-binding protein 1 modulates adiposity and energy homeostasis

作者：Ro Hyo Sung^*; Zhang Lei; Majdalawieh Amin; Kim Sung Woo; Wu Xue; Lyons Peter J; Webber Chris; Ma Hong; Reidy Shannon P; Boudreau Aaron; Miller Jessica R; Mitchell Patricia; McLeod Roger S

来源：Obesity, 2007, 15(2): 288-302.

DOI：10.1038/oby.2007.569

• adipose tissue
• energy balance
• mouse models
• phosphatase and tensin homolog deleted on chromosome ten
• mitogen-activated protein kinase

Objective: To determine whether adipocyte enhancer binding protein (AEBP) 1, a transcriptional repressor that is down-regulated during adipogenesis, functions as a critical regulator of adipose tissue homeostasis through modulation of phosphatase and tensin homolog deleted on chromosome ten (PTEN) tumor suppressor activity and mitogen-activated protein kinase (MAPK) activation.
Research Methods and Procedures: We examined whether AEBP1 physically interacts with PTEN in 3T3-L1 cells by coimmunoprecipitation analysis. We generated AEBP1-null mice and examined the physiological role of AEBP1 as a key modulator of in vivo adiposity. Using adipose tissue from wild-type and AEBP1-null animals, we examined whether AEBP1 affects PTEN protein level.
Results: AEBP1 interacts with PTEN, and deficiency of AEBP1 increases adipose tissue PTEN mass. AEBP1-null mice have reduced adipose tissue mass and enhanced apoptosis with suppressed survival signal. Primary pre-adipocytes from AEBP1-null adipose tissues exhibit lower basal MAPK activity with defective proliferative potential. AEBP1-null mice are also resistant to diet-induced obesity, suggesting a regulatory role for AEBP1 in energy homeostasis.
Discussion: Our results suggest that AEBP1 negatively regulates adipose tissue PTEN levels, in conjunction with its role in proliferation and differentiation of pre-adipocytes, as a key functional role in modulation of in vivo adiposity.

• 出版日期2007-2