Antinociceptive Effect of Glyasperin F Isolated from Glycyrrhiza inflata in Mice

作者：Kim Jin Kyu; Oh Joa Sub; Lee Jin Koo^*

来源：Journal of the Korean Society for Applied Biological Chemistry, 2013, 56(5): 541-545.

DOI：10.1007/s13765-013-3161-2

• antinociception
• formalin test
• glyasperin F
• Glycyrrhiza inflata
• writhing test

Antinociceptive effect of glyasperin F isolated from Glycyrrhiza inflata extract (GM) in ICR mice was studied. Oral administration of GM (1-100 mg/kg) caused a dose-dependent reduction in acetic acid-induced writhing responses. To identify the active antinociceptive compound from the GIE, sub-fractions were obtained from the EtOAc layer of GIE by using a medium pressure liquid chromatography. From the sub-fractions obtained, the sub-fraction, which, when administered orally (10 mg/kg) showed an antinociceptive effect in both the writhing test and second phase of the formalin test was identified as glyasperin F using NMR and MS analyses. Finally, the antinociceptive effect of glyasperin F in mouse models of pain was confirmed. Orally administered glyasperin F (0.1-10 mg/kg) showed a dose-dependent antinociceptive effect in both the writhing test and second phase of the formalin test. Taken together, glyasperin F isolated from the GM may be used as a leading compound for further studies on pain and as a new drug derived from natural products for pain therapy.

• 出版日期2013-10