A molluscan calreticulin ortholog from Haliotis discus discus: Molecular characterization and transcriptional evidence for its role in host immunity

作者：Udayantha H M V; Godahewa G I; Bathige S D N K; Wickramaarachchi W D Niroshana; Umasuthan Navaneethaiyer; De Zoysa Mahanama; Jeong Hyung Bok; Lim Bong Soo^*; Lee Jehee^*

来源：Biochemical and Biophysical Research Communications, 2016, 474(1): 43-50.

DOI：10.1016/j.bbrc.2016.04.056

• Calreticulin
• Genomic structure
• Bacterial and viral challenge
• In silco analysis
• Disk abalone

Calreticulin (CALR), a Ca2+ binding chaperone of the endoplasmic reticulum (ER) and mainly involved in Ca2+ storage and signaling. In this study, we report the molecular characterization and immune responses of CALR homolog from disk abalone (AbCALR). The full length AbCALR cDNA (1837 bp) had an ORF of 1224 bp. According to the multiple alignments analysis, N- and P-domains were highly conserved in all the selected members of CALRs. In contrast, the C-domain which terminated with the characteristic ER retrieval signal (HDEL) was relatively less conserved. The phylogenetic analysis showed that all the selected molluscan homologs clustered together. Genomic sequence of AbCALR revealed that cDNA sequence was dispersed into ten exons interconnected with nine introns. AbCALR mRNA expression shows the significant (P < 0.05) up-regulation of AbCALR transcripts in hemocytes upon bacterial (Listeria monocytogenes and Vibrio parahaemolyticus), viral (Viral haemorrhagic septicaemia virus; VHSV) and immune stimulants (LPS and poly I:C) challenges at middle and/or late phases. These results collectively implied that AbCALR is able to be stimulated by pathogenic signals and might play a potential role in host immunity.

• 出版日期2016-5-20