ADSORPTION OF Candida albicans IN RESINS USED FOR MANUFACTURE OF REMOVABLE PROSTHESIS BASES

作者：Felix da Silva Ramon Ferreira; Gomes Martins Carlos Henrique; Chicaro Vinholis Adriana Helena; Aguiar dos Reis Rodrigo Sant' Anna; Marangoni Soraia; de Souza Talita Telles; Barbosa Anaisa Leal; Casemiro Luciana Assirati

来源：Bioscience Journal, 2013, 29(3): 767-773.

• Acrylic resin
• Removable prosthesis
• Candida albicans
• Microbiology

Heat-activated resin has been selected as material for the preparation of removable prosthesis basis for over 60 years. Microwave-activated and semi-rigid resins have been introduced more recently; however, little is known about their ability to retain biofilms, especially in the case of semi-rigid materials. Therefore, this study evaluated C. albicans adsorption onto four resins employed in removable prosthesis basis. The materials Onda-Cryl (Classic, microwave polymerization), QC-20 (Dentsply, short-cycle heat-activated polymerization), Lucitone (Denstply, heat-activated polymerization), and Odontoflex (Odontoloflex, thermally injected polyamide resin) were investigated. Ten specimens (10.0X5.0X2.0mm) were prepared for each material, following the manufacturer's instructions. The samples were sterilized (121 degrees C, 25min, 1 atm), placed in tubes containing 10.0 mL of RPMI broth (Inlab) inoculated with C. albicans ATCC 28366 (10(6) CFU/mL), and incubated in a shaker (48h/37 degrees C). Next, the specimens were transferred to other test tubes and washed with sterilized distilled water (10s), and were then placed in RPMI broth, under agitation (10s). Aliquots of the RPMI broth were seeded in solid agar Sabouraud culture medium (Difco), which was then incubated (48h/37 degrees C). The resulting colonies were counted, and the data were analyzed (Anova One-Way, Tukey, p <= 0.05). On the basis of C. albicans ATCC 28366 adsorption, the materials were classified in the following decreasing order: 1) Odontoflex (690,000CFU/mL), 2) Onda-Cryl (454,000CFU/mL), 3) Lucitone 550 (256,000CFU/mL), and 4) QC-20 (254,000CFU/mL). Odontoflex and Onda-Cryl were significantly different (p <= 0.05), and they were also statistically different from the other tested materials. Lucitone 550 and QC-20 were similar in terms of yeast adsorption. In conclusion, larger and smaller C. albicans ATCC 28366 retention were verified for the polyamide resin and the heat-activated resin QC 20, respectively.

• 出版日期2013-6