A solid phase-based nanopore sensing system for biomolecules using lipid-loaded mesoporous silica MCM-41 and a fluorescent dye

作者：Takeuchi Ryo; Shoji Atsushi; Sugawara Masao^*

来源：Sensors and Actuators B: Chemical , 2013, 181: 29-37.

DOI：10.1016/j.snb.2013.01.039

• Mesoporous silica material
• Solid phase fluorometry
• Fluorescent dyes
• Lipid-loaded nanopores
• BSA
• Anti-BSA antibody

We describe a nanopore sensing system for biomolecules by using the nanopores of lipid-loaded, receptor-modified mesoporous silica MCM-41 and a fluorescence dye. The pores of MCM-41 are loaded with a lipid mixture, i.e., DOPC(C-18:1):DOPE(C-18:1)=9:1 in a molar ratio. BSA as a receptor is modified via the amine moiety of DOPE(C-18:1). Upon binding to anti-BSA antibody in a solution in the presence of Rhodamine B (RB) as a fluorescent marker, the amount of RB going into the lipid-loaded nanopores is decreased. The solid phase-based fluorometric measurement of the sediment of the bovine serum albumin (BSA)-modified MCM-41 could determine anti-BSA antibody in the concentration range from 1.0 x 10(-10) to 1.0 x 10(-7) g mL(-1) with detection limit of 1.0 x 10(-11) gmL(-1) (S/N). The high sensitivity of the nanopore sensing system was ascribed to the amplified feature of an ion-flux and the enhancement of fluorescence of RB in the pores.

• 出版日期2013-5