Detection of Zearalenone and Its Metabolites in Naturally Contaminated Porcine Follicular Fluid by Using Liquid Chromatography-Tandem Mass Spectrometry

作者：Sambuu Rentsenkhand; Takagi Mitsuhiro^*; Shiga Satoshi; Uno Seiichi; Kokushi Emiko; Namula Zhao; Otoi Takeshige; Miyamoto Akio; Deguchi Eisaburo; Fink Gremmels Johanna

来源：Journal of Reproduction and Development, 2011, 57(2): 303-306.

DOI：10.1262/jrd.10-106M

• Follicular fluid
• LC/MS/MS
• Swine
• Zearalenone

Zearalenone (ZEN) and its metabolites are important nonsteroidal estrogenic mycotoxins that cause reproductive disorders in domestic animals, especially pigs. We aimed to simultaneously detect ZEN and its metabolites alpha-zearalenol (alpha-ZOL) and beta-zearalenol (beta-ZOL) in porcine follicular fluid (FF) by liquid chromatography-tandem mass spectrometry. ZEN and alpha-ZOL, but not beta-ZOL, were detected in all pooled FF samples collected from coexisting follicles (diameter >= 6 mm) within 10 ovaries. Furthermore, ZEN and alpha-ZOL were detected in samples pretreated with beta-glucuronidase/arylsulfatase, but not in those left untreated, suggesting that the FF samples contained glucuronide-conjugated forms of the mycotoxins that may be less harmful to porcine oocytes due to glucuronidation affecting the receptor binding. Nonetheless, the effects of the glucuronide-conjugated forms should be studied, both in vitro and in vivo.

• 出版日期2011-4