A Zyxin-Mediated Mechanism for Actin Stress Fiber Maintenance and Repair

作者：Smith Mark A; Blankman Elizabeth; Gardel Margaret L; Luettjohann Laura; Waterman Clare M^*; Beckerle Mary C

来源：Developmental Cell, 2010, 19(3): 365-376.

DOI：10.1016/j.devcel.2010.08.008

To maintain mechanical homeostasis, cells must recognize and respond to changes in cytoskeletal integrity. By imaging live cells expressing fluorescently tagged cytoskeletal proteins, we observed that actin stress fibers undergo local, acute, force-induced elongation and thinning events that compromise their stress transmission function, followed by stress fiber repair that restores this capability. The LIM protein zyxin rapidly accumulates at sites of strain-induced stress fiber damage and is essential for stress fiber repair and generation of traction force. Zyxin promotes recruitment of the actin regulatory proteins alpha-actinin and VASP to compromised stress fiber zones. alpha-Actinin plays a critical role in restoration of actin integrity at sites of local stress fiber damage, whereas both alpha-actinin and VASP independently contribute to limiting stress fiber elongation at strain sites, thus promoting stabilization of the stress fiber. Our findings demonstrate a mechanism for rapid repair and maintenance of the structural integrity of the actin cytoskeleton.

• 出版日期2010-9-14