Ascaris lumbricoides: Capacidad inhibitoria relacionada al ant赤geno P1 Ascaris lumbricoides: Inhibitory capacity in relation to P1 antigen

作者：Patricia Ponce de Leon; Patricia Foresto; Juana Valverde

来源：Acta bioquˋ-mica clˋ-nica latinoamericana, 2007.

• Ascaris lumbricoides
• capacidad inhibitoria
• antigeno P1
• Ascaris lumbricoides
• inhibitory capacity
• P1 antigen

El Sistema de Grupo Sangu赤neo P tiene un 迆nico ant赤geno: P1 que fue identificado en varias especies par芍sitas. El objetivo de este trabajo fue determinar la capacidad inhibitoria de A. lumbricoides en relaci車n al ant赤geno P1. Se trabaj車 con 11 extractos parasitarios (EA). Para evaluar la capacidad inhibitoria de los extractos en relaci車n a los epitopes P1, se realiz車 la t谷cnica semicuantitativa de inhibici車n de la aglutinaci車n. Se prepararon dos series de diluciones de anticuerpo monoclonal anti- P1; a la primera se le agreg車 PBS a todos los tubos y a la otra, igual volumen de EA. El sistema revelador fue una suspensi車n de eritrocitos P1. Se determin車 el t赤tulo del anticuerpo monoclonal en ambas series. Se consider車 significativa la aglutinaci車n cuando hubo una diferencia de 2 o m芍s diluciones entre ambos t赤tulos. De los 11 EA estudiados, 8 presentaron epitopes P1. A estos 8 extractos se les calcul車 el Poder Inhibitorio y se lo relacion車 con su contenido proteico a los fines de poder comparar la capacidad inhibitoria entre los extractos. La t谷cnica aplicada permiti車 evidenciar la presencia de epitopes del Sistema P en extractos de A. lumbricoides y comparar la capacidad inhibitoria de los extractos en relaci車n a epitopes P1. P Blood Group has a unique antigen: P1. It was identified in various parasite species. The aim of this work was to determine A. lumbricoides∩s Inhibitory Capacity in relation to P1 antigen. The work was based on 11 parasite extracts (AE). The semiquantitative inhibition agglutination technique was used to assess the extracts∩ Inhibitory Capacity in relation to P1 antigen. Two series of anti- P1 monoclonal antibody dilutions were prepared. PBS was added to the first series and an equal volume of AE to the second. Suspensions of P1 red cells were used as a revealing system. Monoclonal antibody titres were determined in both series. The agglutination was considered significative when there was a difference of two or more dilutions between the titres. Eight of the 11 extracts studied presented P1 epithopes. Inhibitory power of these 8 extracts was calculated and it was related with their protein concentrations to compare the Inhibitory Capacity among the extracts. The technique used made it possible to prove P System epithopes presence and to compare the extracts∩ Inhibitory Capacity in relation to P1 epithopes.

• 出版日期2007