Nerve growth factor and brain-derived neurotrophic factor attenuate angiotensin-II-induced facilitation of calcium channels in acutely dissociated nucleus tractus solitarii neurons of the rat

作者：Endoh Takayuki^*; Sato Daisuke; Wada Yoshiyuki; Ishihara Kazuyuki; Hashimoto Sadamitsu; Yoshinari Masao; Matsuzaka Kenichi; Tazaki Masakazu; Inoue Takashi

来源：Archives of Oral Biology, 2008, 53(12): 1192-1201.

DOI：10.1016/j.archoralbio.2008.07.006

• Nerve growth factor
• Brain-derived neurotrophic factor
• Angiotensin II
• Voltage-dependent Ca2+ channel current

Objective: Neurotrophins, such as nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF), promote neuronal development and neuronal survival, but their mechanisms remain controversial. This study aimed to investigate the hypothesis that NGF and BDNF interfere with angiotensin-II-and glutamate-induced facilitation of voltage-dependent Ca2+ channels (VDCCs) in nucleus tractus solitarius (NTS) neurons.
Design: The profile of NGF and BDNF actions in acutely dissociated rat NTS was studied using the whole-cell configuration of the patch-clamp technique.
Results: Pretreatment with NGF and BDNF attenuated angiotensin-II-induced facilitation of VDCCs, but did not attenuate glutamate-induced facilitation of the L-type VDCC current in NTS neurons. NGF-induced attenuation was antagonised by pretreatment with a tyrosine kinase A (TrkA) receptor antagonist K-252a.
Conclusions: NGF attenuated angiotensin-II-induced facilitation of L-type VDCCs mediated by TrkA receptors in NTS neurons.

• 出版日期2008-12