Aberrant expression of signaling proteins in essential thrombocythemia

作者：Hui, Wuhan; Ye, Fei; Zhang, Wei; Liu, Congyan; Cui, Miao; Li, Wei; Xu, Juan^*; Zhang, David Y.

来源：Annals of Hematology, 2013, 92(9): 1229-1238.

DOI：10.1007/s00277-013-1755-8

• Essential thrombocythemia
• Signaling protein
• JAK2V617F mutation
• ER beta
• Stat3

Dysregulated expression of signaling proteins may contribute to the pathophysiology of essential thrombocythemia (ET). This study aimed to characterize protein expression in ET and to correlate the dysregulated proteins with phenotypes and prognosis of ET patients. The expression of 128 proteins in peripheral blood neutrophils from 74 ET patients was assessed and compared with those from 29 healthy subjects and 35 polycythemia vera (PV) patients using protein pathway array. Fifteen proteins were differentially expressed between ET patients and normal controls. These dysregulated proteins were involved in the signaling pathways related with apoptosis and inflammation. Our results showed a significant overlap in protein expression between ET patients with JAK2V617F mutation and PV patients. In addition, nine proteins were associated with JAK2V617F mutation status in ET patients. Furthermore, estrogen receptor beta (ER beta) and Stat3 were independent risk factors for subsequent thrombosis during follow-up on multivariable analysis. Our study shows a broad dysregulation of signaling protein in ET patients, suggesting their roles in ET pathogenesis. The expression levels of ER beta and Stat3 could be promising predictors of subsequent thrombosis in ET patients.

• 出版日期2013-9
• 单位吉林大学; 首都医科大学