ANTIBODY-PRODUCTION IN SHARKS AND HUMANS - A ROLE FOR NATURAL ANTIBODIES

作者：MARCHALONIS JJ^*; HOHMAN VS; THOMAS C; SCHLUTER SF

来源：Developmental and Comparative Immunology, 1993, 17(1): 41-53.

DOI：10.1016/0145-305X(93)90014-H

• AUTOANTIBODY
• IGM
• GAMMA-CHAINS
• NATURAL ANTIBODIES
• SHARKS
• EVOLUTION
• B-CELLS
• IMMUNOGLOBULIN SEQUENCE

Although gene segments specifying Igs of all vertebrates show clear homology, their arrangements differ markedly, thereby suggesting that the mechanisms for the generation of diversity and for the regulation of gene expression may be quite distinct. In the sandbar shark, light chain gene segments are distributed as apparently independent clusters consisting of V, J, and C elements that require rearrangement for expression. The usual distance between V and C in the clusters is 3 kb but larger clusters occur. The V, J, and C elements are clearly homologous to those of human lambda chains. Shark Igs resemble mammalian IgM in structure and gene similarity. IgM may comprise as much as 50% of serum proteins in the shark. By contrast, IgM in humans comprises less than 5%. Human autoantibodies usually are IgM. These show little dependence on thymic function for expression and tend to increase with age. We have carried out a study of the capacity of Igs of unimmunized sharks and people (normals and patients suffering from autoimmune diseases) to react against a panel of antigens, including those usually considered autoantibodies, such as thyroglobulin and single-stranded DNA. Sharks and humans possess IgM antibodies that react with thyroglobulin and ssDNA. Affinity-purified natural shark antibodies to thyroglobulin or ssDNA constitute small fractions of total IgM. They illustrate extensive cross-reactivity comparable to that shown by polyspecific IgM autoantibodies produced by human B cells (CD5+) that appear early in ontogeny.

• 出版日期1993-2