Micron-scale Phenotyping Techniques of Maize Vascular Bundles Based on X-ray Microcomputed Tomography

作者：Zhang, Ying; Ma, Liming; Pan, Xiaodi; Wang, Jinglu; Guo, Xinyu^*; Du, Jianjun^*

来源：Jove-Journal of Visualized Experiments, 2018, (140): e58501.

DOI：10.3791/58501

• Biology
• Issue 140
• Maize
• vascular bundle
• micro-CT
• image analysis pipeline
• three-dimensional construction
• microscopic phenotype

It is necessary to accurately quantify the anatomical structures of maize materials based on high-throughput image analysis techniques. Here, we provide a 'sample preparation protocol' for maize materials (i.e., stem, leaf, and root) suitable for ordinary micro-computed tomography (microCT) scanning. Based on high-resolution CT images of maize stem, leaf, and root, we describe two protocols for the phenotypic analysis of vascular bundles: (1) based on the CT image of maize stem and leaf, we developed a specific image analysis pipeline to automatically extract 31 and 33 phenotypic traits of vascular bundles; (2) based on the CT image series of maize root, we set up an image processing scheme for the three-dimensional (3-D) segmentation of metaxylem vessels, and extracted two-dimensional (2-D) and 3-D phenotypic traits, such as volume, surface area of metaxylem vessels, etc. Compared with traditional manual measurement of vascular bundles of maize materials, the proposed protocols significantly improve the efficiency and accuracy of micron-scale phenotypic quantification.

• 出版日期2018-10
• 单位北京市农林科学院