Anticancer activities of an antimicrobial peptide derivative of Ixosin-B amide

作者：Hsiao Yu Cheng; Wang Kai Shiuan; Tsai Shu Huai; Chao Wei Ting; Lung Feng Di T^*

来源：Bioorganic & Medicinal Chemistry Letters, 2013, 23(20): 5744-5747.

DOI：10.1016/j.bmcl.2013.07.063

• Antimicrobial peptides
• Ixosin-B
• Antimicrobial activity
• Anticancer activity

In nature, antimicrobial peptides (AMPs) represent the first line of defense against infection by pathogens; thus, they are generally good candidates for the development of antimicrobial agents. Recently, we reported two potent antimicrobial peptides, KWLRRVWRWWR-amide (MAP-04-03) and KRLRRVWRRWR-amide (MAP-04-04), which were derived from a fragment of Ixosin-B-amide (KSDVRRWRSRY). Since some cationic AMPs exhibited cytotoxic activity against cancer cells, in the current study, we further investigated the anticancer activity of these potent antimicrobial peptides by antiproliferative assays and wound-healing assays, and the effect of peptide on the cytoskeleton alteration and cell morphology were analyzed by confocal microscopy. Results indicated that MAP-04-03 not only exhibited inhibitory effects on the proliferation (IC50 = 61.5 mu M) and on the cell migration of MCF-7 breast cancer cells (at a concentration of 5 mu M), but also affected the cytoskeleton at the concentration of 25 mu M. These results demonstrated that MAP-04-03 can serve as a lead peptide analog for developing potent anticancer agents.

• 出版日期2013-10-15