Anaerobic Obligatory Xylitol Production in Escherichia coli Strains Devoid of Native Fermentation Pathways

作者:Akinterinwa Olubolaji; Cirino Patrick C*
来源:Applied and Environmental Microbiology, 2011, 77(2): 706-709.
DOI:10.1128/AEM.01890-10

摘要

Anaerobic glucose oxidation was coupled to xylose reduction in a nonfermentative Escherichia coli strain expressing NADPH-dependent xylose reductase. Xylitol production serves as the primary means of NAD(P)(+) regeneration, as glucose is converted primarily to acetate and CO2. The membrane-bound transhydrogenase PntAB is required to achieve the maximum theoretical yield of four moles of xylitol per mole of glucose consumed.

  • 出版日期2011-1