Differential dendritic cell-mediated activation and functions of invariant NKT-cell subsets in oral cancer

作者：Singh A K; Gaur P; Shukla N K; Das S N^*

来源：Oral Diseases, 2015, 21(1): E105-E113.

DOI：10.1111/odi.12238

• iNKT subsets
• -galactosylceramide
• cytokine
• dendritic cells
• cytotoxicity
• oral cancer

ObjectivesInvariant natural killer T (iNKT) cells are unique subset of glycolipid-reactive T lymphocytes with potent antitumour characteristics. This study was planned to understand Th-like cytokine profiles of iNKT-cell subsets and modulation of their functions in response to glycolipid ligand and tumour cell lysate (TL).
Subjects and MethodsCytokine profile of iNKT-cell subsets was evaluated from the peripheral blood of eight oral squamous cell carcinoma (OSCC) patients by flow cytometry and enzyme-linked immunosorbent assay (ELISA), while antitumour activity of iNKT cells was measured by methyl tetrazolium salt assay.
ResultsCD4(+) (CD4(+)CD8(-)) iNKT subset from OSCC patients showed significant (P<0.01) expansion and higher IL-4 production following activation with -GalCer-pulsed DCs, while CD4(-)CD8(-) double negative (DN) and CD8(+) (CD4(-)CD8(+)) iNKT subsets produced IFN- predominantly. iNKT cells showed significantly (P=0.02) increased secretion of IFN- and enhanced cytotoxicity to KB and SCC-4 tumour cells in response to -GalCer and TL-pulsed DCs.
ConclusionIt appears that mutual balance/ratio of iNKT subsets may be important for their effector functions. Selectively expanded DN and CD8(+) iNKT cells with -GalCer and TL may be a better candidate vaccine for iNKT-cell-based adoptive cancer immunotherapy.

• 出版日期2015-1
• 单位河北医科大学