Promotion of Hendra Virus Replication by MicroRNA 146a

作者：Stewart Cameron R^*; Marsh Glenn A; Jenkins Kristie A; Gantier Michael P; Tizard Mark L; Middleton Deborah; Lowenthal John W; Haining Jessica; Izzard Leonard; Gough Tamara J; Deffrasnes Celine; Stambas John; Robinson Rachel; Heine Hans G; Pallister Jackie A; Foord Adam J; Bean Andrew G; Wang Lin Fa

来源：Journal of Virology, 2013, 87(7): 3782-3791.

DOI：10.1128/JVI.01342-12

Hendra virus is a highly pathogenic zoonotic paramyxovirus in the genus Henipavirus. Thirty-nine outbreaks of Hendra virus have been reported since its initial identification in Queensland, Australia, resulting in seven human infections and four fatalities. Little is known about cellular host factors impacting Hendra virus replication. In this work, we demonstrate that Hendra virus makes use of a microRNA (miRNA) designated miR-146a, an NF-kappa B-responsive miRNA upregulated by several innate immune ligands, to favor its replication. miR-146a is elevated in the blood of ferrets and horses infected with Hendra virus and is upregulated by Hendra virus in human cells in vitro. Blocking miR-146a reduces Hendra virus replication in vitro, suggesting a role for this miRNA in Hendra virus replication. In silico analysis of miR-146a targets identified ring finger protein (RNF) 11, a member of the A20 ubiquitin editing complex that negatively regulates NF-kappa B activity, as a novel component of Hendra virus replication. RNA interference-mediated silencing of RNF11 promotes Hendra virus replication in vitro, suggesting that increased NF-kappa B activity aids Hendra virus replication. Furthermore, overexpression of the I kappa B superrepressor inhibits Hendra virus replication. These studies are the first to demonstrate a host miRNA response to Hendra virus infection and suggest an important role for host miRNAs in Hendra virus disease.

• 出版日期2013-4
• 单位CSIRO; 河北医科大学