Monitoring the susceptibility to oseltamivir of Influenza A(H1N1) 2009 virus by nested-PCR and pyrosequencing during the pandemic and in the season 2010-2011

作者：Arvia Rosaria; Corcioli Fabiana; Simi Lisa; Orlando Claudio; De Santis Riccardo; Facchini Marzia; Donatelli Isabella; Azzi Alberta^*

来源：Journal of Virological Methods, 2012, 184(1-2): 113-116.

DOI：10.1016/j.jviromet.2012.05.018

• Antiviral resistance
• Influenza virus
• Pyrosequencing
• Nested PCR

For the early detection of the H275Y mutation as a marker of oseltamivir resistance in A(H1N1) pandemic strains, a sensitive and specific pyrosequencing assay was developed. This assay analyses a region 99 nts long, encompassing the H275Y site, amplified by a nested PCR. Seventy-five respiratory specimens, obtained from 62 patients during the pandemic and in the 2010-2011 influenza season, in Tuscany, were tested. Resistant strains were demonstrated in 10 patients. In three other patients, resistant and sensitive variants were found. This pyrosequencing assay may be a useful method for monitoring the spread of resistant influenza H1N1 2009 strains.

• 出版日期2012-9