MS23, a master basic helix-loop-helix factor, regulates the specification and development of the tapetum in maize

作者：Nan, Guo-Ling^*; Zhai, Jixian; Arikit, Siwaret; Morrow, Darren; Fernandes, John; Mai, Lan; Nhi Nguyen; Meyers, Blake C.; Walbot, Virginia^*

来源：Development, 2017, 144(1): 163-172.

DOI：10.1242/dev.140673

• Pre-meiotic male reproduction
• Regulatory hierarchy
• Transcriptome
• Proteome

Successful male gametogenesis involves orchestration of sequential gene regulation for somatic differentiation in pre-meiotic anthers. We report here the cloning of Male Sterile23 (Ms23), encoding an anther-specific predicted basic helix-loop-helix (bHLH) transcription factor required for tapetal differentiation; transcripts localize initially to the precursor secondary parietal cells then predominantly to daughter tapetal cells. In knockout ms23-ref mutant anthers, five instead of the normal four wall layers are observed. Microarray transcript profiling demonstrates a more severe developmental disruption in ms23-ref than in ms32 anthers, which possess a different bHLH defect. RNA-seq and proteomics data together with yeast two-hybrid assays suggest that MS23 along with MS32, bHLH122 and bHLH51 act sequentially as either homo-or heterodimers to choreograph tapetal development. Among them, MS23 is the earliest-acting factor, upstream of bHLH51 and bHLH122, controlling tapetal specification and maturation. By contrast, MS32 is constitutive and independently regulated and is required later than MS23 in tapetal differentiation.

• 出版日期2017-1-1
• 单位南方科技大学