Ambidextrous magnetic nanovectors for synchronous gene transfection and labeling of human MSCs

作者：Yang Jaemoon; Lee Eun Sook; Noh Min Young; Koh Seong Ho; Lim Eun Kyung; Yoo A Rum; Lee Kwangyeol; Suh Jin Suck; Kim Seung Hyun; Haam Seungjoo^*; Huh Yong Min

来源：Biomaterials, 2011, 32(26): 6174-6182.

DOI：10.1016/j.biomaterials.2011.04.007

• Magnetic
• Nanovectors
• Gene delivery
• Stem cell
• Solvent shifting

The synchronization of gene expression and cell trafficking in transfected stem cells is crucial for augmentation of stem cell functions (differentiation and neurotropic factor secretion) and real time in vivo monitoring. We report a magnetic nanoparticle-based gene delivery system that can ensure simultaneous gene delivery and in vivo cell trafficking by high resolution MR imaging. The polar aprotic solvent soluble MnFe(2)O(4) nanoparticles were enveloped using cationic polymers (branched polyethyleneimine, PEI) by the solvent shifting method for a gene loading. Using our magnetic nanovector system (PEI-coated MnFe(2)O(4) nanoparticles), thus, we synchronized stem cell migration and its gene expression in a rat stroke model.

• 出版日期2011-9