Phenethyl isothiocyanate decreases thymic stromal lymphopoietin-induced inflammatory reactions in mast cells

作者：Han Na-Ra; Moon Phil-Dong; Ryu Ka-Jung; Kim Hyung-Min^*; Jeong Hyun-Ja^*

来源：Journal of Food Biochemistry, 2018, 42(1): e12449.

DOI：10.1111/jfbc.12449

• IL-13
• inflammation
• mast cells
• phenethyl isothiocyanate
• thymic stromal lymphopoietin

Phenethyl isothiocyanate (PEITC) has been reported to have anti-inflammatory and anti-carcinogenic properties. However, the anti-inflammatory effects of PEITC on thymic stromal lymphopoietin (TSLP)-induced inflammatory responses are uncertain. This study evaluates pharmacological activities of PEITC on inflammatory reactions in TSLP-stimulated mast cells. Human mast cell line HMC-1 was treated with PEITC (0.04, 0.4, and 4 mu M) and subjected to inflammation by TSLP. Our results showed that PEITC significantly attenuated IL-13 and TNF- levels increased by TSLP in HMC-1. PEITC significantly decreased TSLP-promoted HMC-1 proliferation and Ki67 mRNA expression. Protein levels of MDM2 and pSTAT6 increased by TSLP were significantly suppressed by PEITC in HMC-1. In addition, PEITC significantly enhanced protein levels of cleaved poly ADP-ribose polymerase and p53 decreased by TSLP. Based on the effects of PEITC on inflammation and proliferation in this study, it is possible that PEITC is a potential candidate to treat mast cells-mediated inflammatory disorders.
Practical applicationsThis report provides strong evidence that Phenethyl isothiocyanate (PEITC) which is a dietary constituent derived from cruciferous vegetables, may be considered an alternative agent for treatment of mast cells-mediated inflammatory disorders.

• 出版日期2018-2