科研之友
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摘要
In this paper, we discuss the correlation between the reagent temperature and the thermistor sensor outside the chamber. The polymerase chain reaction (PCR) chip employed is constructed on a printed circuit board (PCB) with a heating pattern and a thermistor at the opposite side of the chamber. Since the reaction chamber is made with various films and tapes, a thermocouple could be embedded easily to measure the reagent temperature within the chamber. The parameters for chip control, such as the maximum heating power and the temperature compensation coefficient, were determined using the measured temperatures. The results show that the parameters determined enabled the exact control of the reagent temperature during the polymerase chain reaction.
- 出版日期2016
