ADAMTS-9 expression is up-regulated following transient middle cerebral artery occlusion (tMCAo) in the rat

作者：Reid Martin J^*; Cross Alison K; Haddock Gail; Allan Stuart M; Stock Chris J; Woodroofe M Nicola; Buttle David J; Bunning Rowena A D

来源：Neuroscience Letters, 2009, 452(3): 252-257.

DOI：10.1016/j.neulet.2009.01.058

• ADAMTS-9
• Stroke
• Cerebral ischaemia
• tMCAo

The ADAMTS enzymes (a disintegrin and metalloproteinase with thrombospondin type 1-like motifs) have important roles in central nervous system (CNS) physiology and pathology. This current study aimed to analyse the expression of ADAMTS-9 following transient middle cerebral artery occlusion (tMCAo) in the rat, a model of focal cerebral ischaemia. Using real-time RT-PCR, ADAMTS-9 mRNA was demonstrated to be significantly up-regulated in tMCAo brain tissue compared to sham-operated at 24 h post-ischaemia. The mature form of the ADAMTS-9 protein was only detected by Western blotting in brains subjected to tMCAo at 24 h. In situ hybridisation demonstrated that ADAMTS-9 mRNA was expressed by neurones in tMCAo tissue. This study indicates that ADAMTS-9 expression is modulated in response to cerebral ischaemia in vivo and further research will resolve whether it plays a role in the subsequent degenerative or repair processes.

• 出版日期2009-3-20