Age-Related Changes in Scavenger Receptor-Mediated Endocytosis in Rat Liver Sinusoidal Endothelial Cells

作者：Simon Santamaria Jaione; Malovic Ivana; Warren Alessandra; Oteiza Ana; Le Couteur David; Smedsrod Bard; McCourt Peter; Sorensen Karen Kristine^*

来源：Journals of Gerontology Series A-Biological Sciences and Medical Sciences, 2010, 65(9): 951-960.

DOI：10.1093/gerona/glq108

• Aging
• Hepatic sinusoid
• Porosity
• Stabilin
• Scavenger endothelial cells

Liver sinusoidal endothelial cells (LSECs) play an essential role in systemic waste clearance by effective endocytosis of blood-borne waste macromolecules. We aimed to study LSECs' scavenger function during aging, and whether age-related morphological changes (eg, defenestration) affect this function, in F344/BN F1 rats. Endocytosis of the scavenger receptor ligand formaldehyde-treated serum albumin was significantly reduced in LSECs from old rats. Ligand degradation, LSEC protein expression of the major scavenger receptors for formaldehyde-treated serum albumin endocytosis, stabilin-1 and stabilin-2, and their staining patterns along liver sinusoids, was similar at young and old age, suggesting that other parts of the endocytic machinery are affected by aging. Formaldehyde-treated serum albumin uptake per cell, and cell porosity evaluated by electron microscopy, was not correlated, indicating that LSEC defenestration is not linked to impaired endocytosis. We report a significantly reduced LSEC endocytic capacity at old age, which may be especially important in situations with increased circulatory waste loads.

• 出版日期2010-9