Amniotic fluid metabolomics and biochemistry analysis provides novel insights into the diet-regulated foetal growth in a pig model

作者：Wan, Jin; Jiang, Fei; Zhang, Jiao; Xu, Qingsong; Chen, Daiwen; Yu, Bing; Mao, Xiangbing; Yu, Jie; Luo, Yuheng; He, Jun^*

来源：Scientific Reports, 2017, 7(1): 44782.

DOI：10.1038/srep44782

• CHITOSAN OLIGOSACCHARIDE
• AMINO-ACIDS
• INTESTINAL DEVELOPMENT
• BIOLOGICAL-ACTIVITIES
• ANTIOXIDANT CAPACITY
• GESTATIONAL-AGE
• SUCKLING RATS
• WEANED PIGS
• PERFORMANCE
• SPERMINE

Foetal loss and intrauterine growth restriction are major problems in mammals, but there are few effective ways in preventing it. Intriguingly, chitosan oligosaccharide (COS), a biomaterial derived from chitosan, can promote foetal survival and growth. Therefore, we have investigated how COS affects foetal survival and growth in a pig model. Fifty-two sows were divided into two treatment groups (n = 26) and fed either solely a control diet or a control diet that includes 100 mg/kg COS. Amniotic fluid and foetus samples from six sows that were of average body weight in each group were collected on gestation day 35. We applied a H-1 NMR-based metabolomics approach combined with biochemistry analysis to track the changes that occurred in the amniotic fluid of pregnant sows after COS intervention. Maternal COS inclusion had enhanced (P < 0.05) the foetal survival rate and size at 35 days. COS supplementation had both increased (P < 0.05) SOD, CAT and T-AOC activities and elevated (P < 0.05) IL-10, IgG and IgM concentrations in the amniotic fluid. Moreover, COS had affected (P < 0.05) the amniotic fluid's lysine, citrate, glucose and hypoxanthine levels. Overall, COS inclusion induced amniotic fluid antioxidant status and metabolic profiles modifications characterising improvements in foetal survival and growth in a pig model.

• 出版日期2017-3-16
• 单位大连海洋大学; 四川农业大学