摘要
The acaricidal activities of materials derived from Pyrus ussuriensis fruits were evaluated against Tyrophagus putrescentiae and compared with that of commercial acaricide (benzyl benzoate). On the basis of the 50% lethal dose (LD50) values, the ethyl acetate fraction of the fractions obtained from an aqueous extract of P. ussuriensis fruits had the highest acaricidal activity (16.32 mu g/cm(2)) against T. putrescentiae. The acaricidal constituent of P. ussuriensis fruits was isolated by chromatographic techniques and identified as 1,4-benzoquinone. On the basis of the LD50 values, 1,4-benzoquinone (1.98 mu g/cm(2)) was 5.9 times more toxic than benzyl benzoate (11.69 mu g/cm(2)), followed by 2-isopropyl-5-methyl-1,4-benzoquinone (3.29 mu g/cm(2)), and 2,3-dimethoxy-5-methyl-1,4-benzoquinone (5.03 mu g/cm(2)) against T. putrescentiae in the fumigant bioassay. In a filter paper bioassay, the acaricidal activity of 1,4-benzoquinone (0.07 mu g/cm(2)) was 120.1 times more effective than that of benzyl benzoate (8.41 mu g/cm(2)), followed by 2-isopropyl-5-methyl-1,4-benzoquinone (0.11 mu g/cm(2)) and 2,3-dimethoxy-5-methyl-1,4-benzoquinone (0.30 mu g/cm(2)) against T. putrescentiae. These results demonstrate that P. ussuriensis fruit derived material and its derivatives have potential as new preventive agents for the control of stored-food mites.
- 出版日期2012-7