Hyalocyte proliferation and ECM accumulation modulated by bFGF and TGF-beta 1

作者：Sommer Florian; Pollinger Klaus; Brandl Ferdinand; Weiser Barbara; Tessmar Joerg; Blunk Torsten; Goepferich Achim

来源：Graefes Archive for Clinical and Experimental Ophthalmology, 2008, 246(9): 1275-1284.

DOI：10.1007/s00417-008-0846-z

• hyalocyte
• transforming growth factor beta (TGF-beta)
• basic fibroblast growth factor (bFGF)
• cell-based vitreous substitute
• tissue engineering

Purpose In cases of severe retinal diseases, the vitreous body has to be removed and replaced by a suitable biomaterial. Currently, however, no satisfying long-term vitreous substitute is in clinical use. A novel therapeutic concept represents the combination of hyalocytes with suitable biomaterials. The goal of the present study was to evaluate the potential of bFGF and TGF-beta 1 as tools to control hyalocyte proliferation and the accumulation of extracellular matrix (ECM).
Methods In vitro investigation on the influence of different concentrations of bFGF and TGF-beta 1 on hyalocyte morphology, proliferation and ECM production.
Results Both growth factors affected hyalocyte morphology; small, round cells could be observed after bFGF supplementation, whereas the cells appeared more completely spread when cultured with TGF-beta 1. Hyalocyte proliferation was increased 3-fold by 10 ng/ml bFGF; 1 ng/ml TGF-beta 1 in contrast reduced cell proliferation to about 40% of the control. Converse effects of the growth factors could also be observed on the ECM accumulation of hyalocytes; whereas bFGF halved ECM accumulation, TGF-beta 1 enhanced the ECM production up to 3-fold. Precultivation of hyalocytes with bFGF for two passages had no influence on their subsequent accumulation of glycosaminoglycans (GAG). However, cells precultivated with bFGF exhibited a doubled accumulation of collagen compared to controls.
Conclusions The observed opposite effects of bFGF and TGF-beta 1 on hyalocyte proliferation and ECM accumulation may allow for the control of hyaloycte properties. Therefore, these two growth factors seem to be valuable tools towards the development of a cell-based vitreous substitute.

• 出版日期2008-9