A Synthetic Receptor for Asymmetric Dimethyl Arginine

作者：James Lindsey I; Beaver Joshua E; Rice Natalie W; Waters Marcey L^*

来源：Journal of the American Chemical Society, 2013, 135(17): 6450-6455.

DOI：10.1021/ja307907p

Dynamic combinatorial chemistry was utilized to identify a novel small molecule receptor, A(2)D, for asymmetric dimethyl arginine (aRMe(2)), which is a post-translational modification (PTM) in proteins. It is known to play a role in a number of diseases, including spinal muscular atrophy, leukemia, lymphoma, and breast cancer. The receptor exhibits 2.5-7.5-fold selectivity over the isomeric symmetric dimethyl arginine, depending on the surrounding sequence, with binding affinities in the low micromolar range. The affinity and selectivity of A(2)D for the different methylated states of Arg parallels that of proteins that bind to these PTMs. Characterization of the receptor-PTM complex indicates that cation-pi interactions provide the main driving force for binding, loosely mimicking the binding mode found in the recognition of dimethyl arginine by native protein receptors.

• 出版日期2013-5-1