Acetylated histone H3 and H4 mark the upregulated LMP2A promoter of Epstein-Barr virus in lymphoid cells

作者：Gerle Borbala; Koroknai Anita; Fejer Gyoergy; Bakos Agnes; Banati Ferenc; Szenthe Kalman; Wolf Hans; Niller Hans Helmut; Minarovits Janos; Salamonl Daniel^*

来源：Journal of Virology, 2007, 81(23): 13242-13247.

DOI：10.1128/JVI.01396-07

We analyzed the levels of acetylated histones and histone H3 dimethylated on lysine 4 (H3K4me2) at the LMP2A promoter (LMP2Ap) of Epstein-Barr virus in well-characterized type I and type III lymphoid cell line pairs and additionally in the nasopharyngeal carcinoma cell line C666-1 by using chromatin immunoprecipitation. We found that enhanced levels of acetylated histones marked the upregulated LMP2Ap in lymphoid cells. In contrast, in C666-1 cells, the highly DNA-methylated, inactive LMP2Ap was also enriched in acetylated histones and H3K4me2. Our results suggest that the combinatorial effects of DNA methylation, histone acetylation, and H3K4me2 modulate the activity of LMP2Ap.

• 出版日期2007-12