Hoxa9 Collaborates with E2A-PBX1 in Mouse B Cell Leukemia in Association With Flt3 Activation and Decrease of B Cell Gene Expression

作者：Hassawi Mona; Shestakova Elena A; Fournier Marilaine; Lebert Ghali Charles Etienne; Vaisson Gratianne; Frison Heloise; Sinnett Daniel; Vidal Ramon; Thompson Alexander; Bijl Janet J^*

来源：Developmental Dynamics, 2014, 243(1): 145-158.

DOI：10.1002/dvdy.24056

• Hox genes
• oncogenes
• transgenic mouse model
• transcription factors

Background: The fusion protein E2A-PBX1 induces pediatric B cell leukemia in human. Previously, we reported oncogenic interactions between homeobox (Hox) genes and E2A-PBX1 in murine T cell leukemia. A proviral insertional mutagenesis screen with our E2A-PBX1 B cell leukemia mouse model identified Hoxa genes as potential collaborators to E2A-PBX1. Here we studied whether Hoxa9 could enhance E2A-PBX1 leukemogenesis. Results: We show that Hoxa9 confers a proliferative advantage to E2A-PBX1 B cells. Transplantation experiments with E2A-PBX1 transgenic B cells overexpressing Hoxa9 isolated from bone marrow chimeras showed that Hoxa9 accelerates the generation of E2A-PBX1 B cell leukemia, but Hoxa9 is unable to transform B cells alone. Quantitative-reverse transcriptase polymerase chain reaction analysis demonstrated a strong repression of B cell specific genes in these E2A-PBX1/Hoxa9 leukemias in addition to Flt3 activation, indicating inhibition of B cell differentiation in combination with enhanced proliferation. Overexpression of Hoxa9 in established E2A-PBX1 mouse leukemic B cells resulted in a growth advantage in vitro, which was also characterized by an enhanced expression of Flt3. Conclusions: we show for the first time that Hoxa9 collaborates with E2A-PBX1 in the oncogenic transformation of B cells in a mouse model that involves Flt3 signaling, which is potentially relevant to human disease. Developmental Dynamics 243:145-158, 2014.

• 出版日期2014-1