Altered E-NTPDase/E-ADA activities and CD39 expression in platelets of sickle cell anemia patients

作者：Castilhos Livia G^*; Doleski Pedro H; Adefegha Stephen A; Becker Lara V; Ruchel Jader B; Leal Daniela B R^*

来源：Biomedicine & Pharmacotherapy, 2016, 79: 241-246.

DOI：10.1016/j.biopha.2016.02.009

• Sickle cell anemia
• Platelet
• CD39
• CD73
• Adenosine deaminase

Sickle cell anemia (SCA) is a hemoglobinopathy characterized by hemolysis and vaso-occlusions caused by rigidly distorted red blood cells. Sickle cell crisis is associated with extracellular release of nucleotides and platelets, which are critical mediators of hemostasis participating actively in purinergic thromboregulatory enzymes system. This study aimed to investigate the activities of purinergic system ecto-enzymes present on the platelet surface as well as CD39 and CD73 expressions on platelets of SCA treated patients. Fifteen SCA treated patients and 30 health subjects (control group) were selected. Ectonucleoside triphosphate diphosphohydrolase (E-NTPDase), ecto-50-nucleotidase (E-5'-NT) and ectoadenosine deaminase (E-ADA) activities were measured in platelets isolated from these individuals. Results demonstrated an increase of 41 % in the E-NTPDase for ATP hydrolysis, 52% for ADP hydrolysis and 60 % in the E-ADA activity in SCA patients (P < 0.05); however, a two folds decrease in the CD39 expression in platelets was observed in the same group (P < 0.01). The increased E-NTPDase activity could be a compensatory mechanism associated with the low expression of CD39 in platelets. Besides, alteration of these enzymes activities suggests that the purinergic system could be involved in the thromboregulatory process in SCA patients.

• 出版日期2016-4