Molecular profiling of human oocytes after vitrification strongly suggests that they are biologically comparable with freshly isolated gametes

作者：Di Pietro Cinzia^*; Vento Marilena; Guglielmino Maria Rosa; Borzi Placido; Santonocito Manuela; Ragusa Marco; Barbagallo Davide; Duro Laura Rita; Majorana Alessandra; De Palma Adele; Garofalo Maria Rita; Minutolo Elisa; Scollo Paolo; Purrello Michele

来源：Fertility and Sterility, 2010, 94(7): 2804-2807.

DOI：10.1016/j.fertnstert.2010.04.060

• Human oocytes
• gene expression
• molecular markers
• vitrification
• real time RT-PCR

To assess the effects of vitrification on the biomolecular profile of oocytes, we analyzed through real-time reverse transcriptase-polymerase chain reaction eight genes encoding critically important proteins for embryo development and compared this partial transcriptome with that of freshly collected gametes isolated from the same women. The comparison of the molecular profiles demonstrated that our vitrification protocol does not alter the biomolecular quality of oocytes: in fact, between the two groups we found the absence of statistically significant variations. Accordingly, this cryopreservation technique might be helpful in preserving women's fertility. (Fertil Steril (R) 2010;94:2804-7.

• 出版日期2010-12