An iron-regulated sortase anchors a class of surface protein during Staphylococcus aureus pathogenesis

作者：Mazmanian SK^*; Ton That H; Su K; Schneewind O

来源：Proceedings of the National Academy of Sciences, 2002, 99(4): 2293-2298.

DOI：10.1073/pnas.032523999

Sortase (SrtA), an enzyme that anchors surface proteins to the cell wail of Gram-positive bacteria, cleaves sorting signals at the LPXTG motif. We have identified a second sortase (SrtB) in the Gram-positive pathogen Staphylococcus aureus that is required for anchoring of a surface protein with a NPQTN motif. Purified SrtB cleaves NPQTN-bearing peptides in vitro, and a srtB mutant is defective in the persistence of animal infections. srtB is part of an iron-regulated locus called iron-responsive surface determinants (isd), which also contains a ferrichrome transporter and surface proteins with NPQTN and LPXTG motifs. Cell wall-anchored surface proteins and the isd locus seem involved in a novel mechanism of iron acquisition that is important for bacterial pathogenesis.

• 出版日期2002-2-19