A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination in chinese agricultural samples

Liu, Yi H.; Chen, Jian; Guo, Yi R.; Wang, Chun M.; Liang, Xiao; Zhu, Guo N.<sup>*</sup>

doi:10.1080/03601234.2011.559884

登录

免费注册

赞收藏引用

科研之友

微信

新浪微博

Facebook

分享链接

A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination in chinese agricultural samples

作者：Liu, Yi H.; Chen, Jian; Guo, Yi R.; Wang, Chun M.; Liang, Xiao; Zhu, Guo N.^*

来源：Journal of Environmental Science and Health - Part B: Pesticides, Food Contaminants, and Agricultural Wastes , 2011, 46(4): 313-320.

DOI：10.1080/03601234.2011.559884

摘要

A monoclonal antibody-based competitive antibody-coated enzyme-linked immunosorbent assay (ELISA) was developed and optimized for determining chlorpyrifos residue in agricultural products. The IC50 and IC10 of this ELISA were 3.3 ng/mL and 0.1 ng/mL respectively. The average recoveries in six agricultural products were between 79.5% and 118.0%, with the intra-assay coefficient of variation being less than 8 %. The limit of detection for all tested products was 30 ng/g. To the best of our knowledge, this assay has the best specificity among all the published research on ELISAs for chlorpyrifos.