Apoptosis triggered by pyropheophorbide-alpha methyl ester-mediated photodynamic therapy in a giant cell tumor in bone

作者：Li, K-T; Zhang, J.; Duan, Q-Q; Bi, Y.; Bai, D-Q^*; Ou, Y-S

来源：Laser Physics, 2014, 24(6): 066002.

DOI：10.1088/1054-660X/24/6/066002

• photodynamic therapy
• pyropheophorbide-alpha methyl ester
• apoptosis
• mitochondrial membrane potential

A giant cell tumor in bone is the common primary bone tumor with aggressive features, occurring mainly in young adults. Photodynamic therapy is a new therapeutic technique for tumors. In this study, we investigated the effects of Pyropheophorbide-alpha methyl ester (MPPa)-mediated photodynamic therapy on the proliferation of giant cell tumor cells and its mechanism of action. Cell proliferation was evaluated using an MTT assay. Cellular apoptosis was detected by Hoechst nuclear staining, and flow cytometric assay. Mitochondrial membrane potential changes and cytochrome c, caspase-9, caspase-3, and Bcl-2 expression was assessed. Finally, we found that MPPa-mediated photodynamic therapy could effectively suppress the proliferation of human giant cell tumor cells and induce apoptosis. The mitochondrial pathway was involved in the MPPa-photodynamic therapy-induced apoptosis.

• 出版日期2014-6
• 单位重庆医科大学