A method to determine the kinetics of multiple proteins in human infants with respiratory distress syndrome

作者：Bereman Michael S^*; Tomazela Daniela M; Heins Hillary S; Simonato Manuela; Cogo Paola E; Hamvas Aaron; Patterson Bruce W; Cole F Sessions; MacCoss Michael J

来源：Analytical and Bioanalytical Chemistry, 2012, 403(8): 2397-2402.

DOI：10.1007/s00216-012-5953-3

• Protein turnover
• Respiratory distress syndrome
• Selected reaction monitoring
• SRM
• Protein kinetics
• Protein metabolism

We report a method to measure in vivo turnover of four proteins from sequential tracheal aspirates obtained from human newborn infants with respiratory distress syndrome using targeted proteomics. We detected enrichment for all targeted proteins approximately 3 h from the start of infusion of [5,5,5-H-2(3)] leucine, secretion times that varied from 1.2 to 2.5 h, and half lives that ranged between 10 and 21 h. Complement factor B, a component of the alternative pathway of complement activation, had an approximately twofold-longer half-life than the other three proteins. In addition, the kinetics of mature and carboxy-terminal tryptic peptides from the same protein (surfactant protein B) were not statistically different (p = 0.49).

• 出版日期2012-6