Superresolution by image scanning microscopy using pixel reassignment

作者:Sheppard Colin J R*; Mehta Shalin B; Heintzmann Rainer
来源:Optics Letters, 2013, 38(15): 2889-2892.
DOI:10.1364/OL.38.002889

摘要

The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied. It is shown how the method can also be employed if there is a Stokes shift in fluorescence emission wavelength. With no Stokes shift, the width of the point spread function can be sharpened by a factor of 1.53, and its peak intensity increased by a factor of 1.84.

  • 出版日期2013-8-1