Allelism and Molecular Marker Tests for Genic Male Sterility in Paprika Cultivars

作者：Lee Jundae; Do Jae Wahng; Han Jung Heon; An Chul Geon; Kweon Oh Yoel; Kim Yong Kwon; Yoon Jae Bok^*

来源：Korean Journal of Horticultural Science and Technology, 2011, 29(2): 130-134.

• allelism test
• GMS
• molecular breeding
• sweet pepper

Paprika (Capsicum annum L.), a colored bell-type sweet pepper, is one of the most important money making vegetable crops in Korea. The cultivation area, total production, and exports of paprika are gradually getting increased, but the paprika cultivars used in Korea are all imported. It was well-known that the genic male sterility (GMS) is the main way to produce paprika hybrid seeds. However, it is little known that how many and what kinds of ins genes are used for breeding of paprika F(1) varieties. In this study, eight paprika cultivars ('Special', 'Debla', 'Plenty', 'Fiero', 'Boogie', 'Fiesta', 'Derby', and 'Minibell'), popularly cultivated in Korea and three different genic male sterile lines ('GMSP', 'GMS3', and 'GMSK') were used. For allelism test among the F(1) cultivars, half diallel crosses were performed. The result demonstrated that the most of the GMS in paprika cultivars except for 'Minibell' were same allele. To identify which GMS gene(s) were used for paprika F(1) cultivars, top crosses between previously known GMS lines and the F(1) cultivars were performed. As a result, we found that the ms(k) and the ms(p) genes were alleles for the GMS of 'Minibell' and for the other cultivars, respectively. We also confirmed that the GMS gene identification using GMSK-CAPS marker linked to the ms(k) gene and the PmsM1-CAPS marker linked to the ms(p) gene in F(2) progenies of 'Minibell' and 'Fiesta' and 'Derby' cultivars, respectively. In addition, we developed the PmsM2-CAPS marker for 'Plenty', 'Fiero', and 'Boogie' cultivars. We expect that these markers will be very useful for breeding new maternal (male sterile) line of paprika.

• 出版日期2011-4