摘要

We reconsider the use of stiffness measurements to estimate N, the number of myosin heads acting (working at any instant to produce tension) on a single actin filament in vertebrate striated muscle, and give reasons for our rejection of numbers produced from such measurements. We go on to present a different approach to the problem, citing and extending a model bearing on the value of N which is derived from other physiological and biochemical data and which offers insight into the fundamental actin myosin contractile event as an impulsive force. New experimental data accumulating over the past decade support this model, in which the myosin heads act sequentially along the actin filament (this is an example of Conformational Spread). In this model only a single myosin head acts on a single actin filament to produce an impulse at any given instant in normally-contracting muscle, either in the isometric or the isotonic mode, so N = 1. However, extra impulses occur within the same time frame after quick release of length or tension. The predictions of this sequential model are in striking agreement with a large body of recent detailed biophysical and biochemical evidence. We suggest that this warrants further in-depth experimental work, specifically to explore and test the sequential model and its implications.

  • 出版日期2012-1

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