A novel synthetic compound, (Z)-5-(3-hydroxy-4-methoxybenzylidene)-2-iminothiazolidin-4-one (MHY773) inhibits mushroom tyrosinase

作者：Jung Hee Jin; Lee Min Jung; Park Yeo Jin; Noh Sang Gyun; Lee A Kyoung; Moon Kyoung Mi; Lee Eun Kyeong; Bang Eun Jin; Park Yun Jung; Kim Su Jeong; Yang Jungho; Ullah Sultan; Chun Pusoon; Jung Young Suk; Moon Hyung Ryong^*; Chung Hae Young^*

来源：Bioscience Biotechnology and Biochemistry, 2018, 82(5): 759-767.

DOI：10.1080/09168451.2018.1445518

• Tyrosinase
• alpha-melanocyte-stimulating hormone(-MSH)
• 3-isobutyl-1-methylxanthine (IBMX)
• MHY773

As part of continued efforts for the development of new tyrosinase inhibitors, (Z)-5-(substituted benzylidene)-2-iminothiazolidin-4-one derivatives (1a - 1l) were rationally synthesized and evaluated for their inhibitory potential in vitro. These compounds were designed and synthesized based on the structural attributes of a -phenyl-,-unsaturated carbonyl scaffold template. Among these compounds, (Z)-5-(3-hydroxy-4-methoxybenzylidene)-2-iminothiazolidin-4-one (1e, MHY773) exhibited the greatest tyrosinase inhibition (IC50 = 2.87 M and 8.06 M for monophenolase and diphenolase), and outperformed the positive control, kojic acid (IC50 = 15.59 and 31.61 M). The kinetic and docking studies demonstrated that MHY773 interacted with active site of tyrosinase. Moreover, a melanin quantification assay demonstrated that MHY773 attenuates -melanocyte-stimulating hormone (-MSH) and 3-isobutyl-1-methylxanthine (IBMX)-induced melanin contents in B16F10 melanoma cells. Taken together, these data suggest that MHY773 suppressed the melanin production via the inhibition of tyrosinase activity. MHY773 is a promising for the development of effective pharmacological and cosmetic agents for skin-whitening.

• 出版日期2018