N-Terminal Pro-C-Natriuretic Peptide and Cytokine Kinetics in Dogs with Endotoxemia

作者：Floras A N K; Holowaychuk M K^*; Bienzle D; Bersenas A M E; Sharif S; Harvey T; Nordone S K; Wood G A

来源：JOURNAL OF VETERINARY INTERNAL MEDICINE, 2014, 28(5): 1447-1453.

DOI：10.1111/jvim.12409

• Biomarker
• ELISA
• Immunology
• Inflammation
• Multiplex
• Sepsis

Background: Serum N-terminal pro-C-natriuretic peptide (NT-proCNP) concentration at hospital admission has sufficient sensitivity and specificity to differentiate naturally occurring sepsis from nonseptic systemic inflammatory response syndrome (SIRS). However, little is known about serum NT-proCNP concentrations in dogs during the course of sepsis. %26lt;br%26gt;Objective: To determine serum NT-proCNP and cytokine kinetics in dogs with endotoxemia, a model of canine sepsis. %26lt;br%26gt;Samples: Eighty canine serum samples. %26lt;br%26gt;Methods: Eight healthy adult Beagles were randomized to receive Escherichia coli lipopolysaccharide (LPS, 5 mu g/kg) or placebo (0.9% NaCl) as a single IV dose in a randomized crossover study. Serum collected at 0, 1, 2, 4, and 24 hours was stored at -80 degrees C for batch analysis. Serum NT-proCNP was measured by ELISA and 13 cytokines and chemokines by multiplex magnetic bead-based assay. %26lt;br%26gt;Results: Serum NT-proCNP concentrations did not differ significantly between LPS-and placebo-treated dogs at any time. When comparing serum cytokine concentrations, LPS-treated dogs had higher interleukin-6 (IL-6), IL-10, TNF-alpha and KC-like at 1, 2, and 4 hours; higher CCL2 at 1, 2, 4, and 24 hours; and higher IL-8 and CXCL10 at 4 hours compared to placebo-treated dogs. There were no differences in serum GM-CSF, IFN-gamma, IL-2, IL-7, IL-15 or IL-18 between LPS-and placebo-treated dogs. %26lt;br%26gt;Conclusions and Clinical Importance: Serum NT-proCNP concentration does not change significantly in response to LPS administration in healthy dogs. Certain serum cytokine and chemokine concentrations are significantly increased within 1-4 hours after LPS administration and warrant further investigation as tools for the detection and management of sepsis in dogs.

• 出版日期2014-10