Acute doxorubicin nephrotoxicity in rats with malignant neoplasm can be successfully treated with fullerenol C-60(OH)(24) Via suppression of oxidative stress

作者：Injac Rade^*; Boskovic Marija; Perse Martina; Koprivec Furlan Eva; Cerar Anton; Djordjevic Aleksandar; Strukelj Borut

来源：Pharmacological Reports, 2008, 60(5): 742-749.

• doxorubicin
• nephrotoxicity
• fullerenol
• oxidative stress
• kidney
• rats
• mammary carcinomas

Oxidative stress has ail important role in the pathogenesis of doxorubicin (DOX)-induced nephrotoxicity. The aim of this Study was to investigate the nephroprotective effects of fullerenol (FLR), an antioxidant agent, on DOX-induced nephrotoxicity. The investigation was carried out on adult female Sprague Dawley outbred rats with chemically induced breast cancer (I -methyl-I-nitrosourea; 50 mg/kg ip). Rats were divided into the following groups: control healthy, control cancer, DOX alone (8 rng/kg, ip, cancer), DOX plus FLR as a pre-treatment (8 mg/kg and 100 rng/kg, respectively, ip, cancer), and FLR alone (100 mg/kg, ip, cancer). At the end of the 2nd day after drug administration, blood and kidney tissues were taken for analysis. The activity of lactate dehydrogenase and (x-hydroxybutyrate dehydrogenase as serum enzymes, as well as level of malondialdehyde, glutathionc. glutathione peroxidase, glutathione reductases, catalase and Superoxide dismutase, were determined. DOX caused nephrotoxicity, but FLR pre-treatment prevented oxidative stress, lipid peroxidation and the disbalance of GSH/GSSG levels in kidney tissue caused by DOX. Our results confirm satisfactory nephroprotective efficacy of FLR in the acute phase of toxicity and encourage further studies regarding its use as a potential nephroprotector.

• 出版日期2008-10