A universal indicator dye pH assay for crystallization solutions and other high-throughput applications

作者:Newman Janet*; Sayle Roger A; Fazio Vincent J
来源:Acta Crystallographica Section D-Biological Crystallography, 2012, 68(8): 1003-1009.
DOI:10.1107/S0907444912018768

摘要

In protein crystallization, as well as in many other fields, it is known that the pH at which experiments are performed is often the key factor in the success or failure of the trials. With the trend towards plate-based high-throughput experimental techniques, measuring the pH values of solutions one by one becomes prohibitively time-and reagent-expensive. As part of an HT crystallization facility, a colour-based pH assay that is rapid, uses very little reagent and is suitable for 96-well or higher density plates has been developed.

  • 出版日期2012-8
  • 单位CSIRO