Development of a simplified method of human semen storage for the testing of sperm DNA fragmentation using the Halosperm G2 test kit

作者：McEvoy Ashleigh^*; Roberts Peter; Yap Kailin; Matson Phillip

来源：Fertility and Sterility, 2014, 102(4): 981-988.

DOI：10.1016/j.fertnstert.2014.07.737

• Halosperm
• semen storage
• sperm DNA damage

Objective: To develop a simple, convenient, and stable storage method for semen before DNA fragmentation testing.
Design: Experimental cross-sectional study.
Setting: Fertility clinic.
Patient(s): 164 male partners of infertile couples.
Intervention(s): Comparison of sperm DNA fragmentation levels (DFLs) using fresh, snap-frozen and air-dried semen, with air-dried samples stored at different temperatures and time periods to assess DNA stability.
Main Outcome Measure(s): DFL determined by Halosperm G2 kit.
Result(s): Results are expressed as mean +/- standard error of the mean. The DFLs from fresh and air-dried semen gave comparable results (1.08% +/- 0.65%), and from snap-frozen and fresh samples a statistically significant difference (5.5% +/- 1.09%). Air-dried semen stored at room temperature for 7 days had a statistically significantly higher DFL compared with semen stored overnight (46.29% +/- 9.12%). Samples stored at 4 degrees C for 7 days or 1 day showed no statistically significant difference (0.83% +/- 0.82%). DFLs from samples stored for either 1 or 30 days at 4 degrees C showed a statistically significant difference (19.59% +/- 5.72%); those stored at -22 degrees C showed no statistically significant difference (0.68% +/- 0.53%).
Conclusion(s): Air-drying semen is a simple and stable storage method for up to 1 month at -22 degrees C before DNA fragmentation testing.

• 出版日期2014-10