A metabonomics study on Crohn%26apos;s Disease using Nuclear Magnetic Resonance spectroscopy

作者：Fathi Fariba; Kyani Anahita; Nejad Mohamad Rostami; Rezaye Tavirani Mostafa; Naderi Nosratollah; Zali Mohamad Reza; Tafazzoli Mohsen^*; Oskouie Afsaneh Arefi

来源：Healthmed, 2012, 6(11): 3577-3583.

• Nuclear magnetic resonance spectroscopy
• Crohn%26apos
• s disease
• Classification and regression tree
• metabonomics

Objective: Crohn%26apos;s disease (CD) is one the important illnesses can affect any part of the gastrointestinal tract. CD is not easily diagnosed using the clinical tests. Thus, the discovery of proper methods would be a major step towards CD diagnosis. The aim of this study was to seek the metabolic biomarkers causes of CD compare to control group. %26lt;br%26gt;Materials and Methods: In present study, we employed metabolic profiling using proton nuclear magnetic resonance spectroscopy (1HNMR) to find metabolites in serum which are helpful for the diagnosis of CD. Classification of CD and healthy subject was done using classification and regression trees (CART). The metabolites that caused changes in people with CD were identified using CART. %26lt;br%26gt;Results: According to CART diagram, we concluded that just using one descriptor CD and control groups could be classified separately. The obtained classification model showed a 89% correct classification of CD and healthy subject for the external test set. %26lt;br%26gt;Conclusions: The findings of the present study reveal new differentiating metabolites for CD. This metabolite may provide diagnostic biomarkers and/or monitoring tools as well as insight into potential targets for prevention and disease therapy.

• 出版日期2012