科研之友
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摘要
Objective: In the present study, we aimed to systematically detect the expression of miR-26b in osteosarcoma patients by qRT-PCR. Meanwhile, we intended to analyze the clinical significance and prognosis value of miR-26b in osteosarcoma patients. Methods: Between June 2007 and February 2015, a total of 107 human osteosarcoma samples and the adjacent non-tumor tissues were collected. The expression of miR-26b was estimated by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). Log-rank test and Kaplan-Meier method were used to evaluate survival rate in patients with osteosarcoma. Multivariate analysis was performed to evaluate prognostic values using Cox proportional hazards model. Results: The expression of miR-26b was significantly lower in osteosarcoma samples compared to non-cancerous samples (P<0.001). Low miR-26b expression level was observed to be closely correlated with distant metastasis (P=0.004), response to chemotherapy (P=0.002), and clinical stage (P=0.001). Kaplan-Meier analysis with the log-rank test indicated that miR-26b expression had a significant impact on overall survival (P=0.004). Multivariate analysis revealed that miR-26b expression level was independent prognostic factor for overall survival (HR=2.187, 95% CI: 1.261-8.012; P=0.019). Conclusion: Our results suggested that miR-26b expression might be associated with aggressive features of osteosarcoma patients. Detection of tissue miR-26b levels might have clinical potentials as a non-invasive prognostic biomarker for osteosarcoma patients.
- 出版日期2016
- 单位华中科技大学
