Apoptosis-inducing Factor, Mitochondrion-associated 2, Regulates Klf1 in a Mouse Erythroleukemia Cell Line

作者：Kojima Naoko; Tanaka Yuka; Kulkeaw Kasem; Nakanishi Yoichi; Shirasawa Senji; Sugiyama Daisuke^*

来源：Anticancer Research, 2015, 35(8): 4493-4499.

• erythropoiesis
• apoptosis-inducing factor
• mitochondrion-associated 2 (Aifm2)
• murine erythroleukemia cells

Background/Aim: Apoptosis-inducing factor, mitochondrion-associated 2 (Aifm2), is a DNA-binding oxoreductase protein that promotes apoptosis. To assess its potential role in erythropoiesis we analyzed the effects of Aifm2 loss-of-function in the murine erythroleukemia line (MEL). Materials and Methods: MEL cells were transfected with siRNA targeting Aifm2 for 24 h and evaluated by cell counting, flow cytometry with annexin V and PI staining and gene expression analysis. Results: Aifm2 knockdown did not affect the apoptotic status of MEL cells. However, Aifm2 knockdown significantly increased expression of the erythropoietic transcription factor Klf1 (2.9+/-0.2-fold, p< 0.05) and decreased alpha- and beta-globin expression (0.6+/-0.2-fold, p< 0.05 and 0.5+/-0.2-fold, p< 0.01). Conclusion: Aifm2 may function in differentiation of erythroid MEL cells in vitro.

• 出版日期2015-8