Urease-catalyzed synthesis of aminocyanopyridines from urea under fully green conditions

作者：Tamaddon Fatemeh^*; Ghazi Somayeh; Noorbala Mohammad Reza

来源：Journal of Molecular Catalysis B: Enzymatic , 2016, 127: 89-92.

DOI：10.1016/j.molcatb.2016.02.015

• Urease
• Enzymatic reactions
• In situ generation of ammonia
• Biocompatible
• Pyridines

This is an original work on catalytic performance of urease in organic synthesis which in one-pot dissociation of urea and condensation of the in situ generated ammonia with aldehydes, acetophenones, and malononitrile occurs in water to give 2-amino-3-cyanopyridines. Comparative experiments with ammonium salts supported the enzymatic specify of 0.01 g/mL (50 U/mg) of urease for bio-production of ammonia, while trace amount of heavy metal ions such as Pb2+, Hg2+, and Ag+ inhibit these specific reactions. The scalability and promiscuity of urease facilitate the applicability of the process for biotechnological organic reactions based on ammonia.

• 出版日期2016-5